T7 RNA POLYMERASE

T7 RNA Polymerase
T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available.
Application: Synthesis of labeled and unlabeled RNA transcripts (1).
Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid.
Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a
transcription reaction.
Unit Definition: One unit hydrolyzes 1 nmol of ribonucleotide into acid-precipitable material in 1 h at 37°C using a T7 transcription
vector as template.
Promoter: T7
Hot Start: No 
Polymerase: T7 RNA Polymerases 
Product Size: 2 x 2,500 units 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
T7 RNA Polymerase is supplied with a vial of 5X reaction buffer [200 mM Tris-HCl (pH 8.0), 40 mM MgCl2 , 10 mM spermidine-(HCl)3 , 125 mM NaCl], vial of 100 mM DTT. Store at -20°C.