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DNA Polymerase I
DNA Polymerase I is a DNA polymerase with 5´→3´ and 3´→5´ exodeoxyribonuclease activities. DNA Polymerase I also incorporates biotinylated nucleotides.
• Applications—DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs
• Source—purified from E. coli expressing the DNA Polymerase I gene on a plasmid
Performance and quality testing
Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.
Unit definition
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.
Hot Start: No
Polymerase: DNA Polymerase I
Product Size: 250 units
Exonuclease Activity: 5' - 3',
3' - 5'
Shipping Condition: Wet Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Store in freezer (-5 to -30°C). |