T4 DNA POLYMERASE

T4 DNA Polymerase
T4 DNA Polymerase is a DNA polymerase that has a 3´-exodeoxyribonuclease activity, but lacks 5´a3´ exodeoxyribonuclease activity. A T4 DNA Polymerase Technical Bulletin is available.
Applications: Labeling double-stranded linear DNA by replacement synthesis (1). Oligonucleotide-directed, site-specific mutagenesis (2). 3´ end-labeling of double-stranded DNA (3). Polishing both 5´ or 3 overhangs to make blunt ends (4).
Source: Purified from E. coli expressing the T4 DNA Polymerase gene on a plasmid.
Performance and Quality Testing: Single- and double-stranded endodeoxyribonuclease and phosphatase assays; exodeoxyribonuclease and polymerase activities tested.
Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using DNase I-nicked DNA as template•primer.
Hot Start: No 
Polymerase: T4 DNA Polymerase 
Product Size: 50 units 
Product Overhang: Blunt 
Exonuclease Activity: 3' - 5' 
Shipping Condition: Wet Ice 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.