S1 NUCLEASE

S1 Nuclease
S1 Nuclease is a single-strand-specific endonuclease that hydrolyzes single-stranded RNA or DNA into 5´ mononucleotides. The enzyme will hydrolyze single-stranded regions in duplex DNA such as loops and gaps. S1 Nuclease is stable at 65°C.
Applications: Nuclease mapping techniques (1,2). Removal of single-stranded regions from double-stranded DNA (3). Exo III-ordered sequencing (4).
Source: Isolated from Aspergillus oryzae.
Performance and Quality Testing: Double-strand-specific deoxyribonuclease and phosphatase assays.
Unit Definition: One unit hydrolyzes 1 µg of denatured DNA to acid-soluble material in 1 min. at 37°C.
Enzyme: Nuclease 
Product Size: 20,000 units 
Shipping Condition: Approved for shipment on Wet or Dry Ice 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
S1 Nuclease is supplied with a vial of 10X S1 Nuclease buffer [300 mM sodium acetate (pH 4.6), 10 mM zinc acetate, 50% (v/v) glycerol], vial of dilution buffer, vial of 3 M NaCl. Store at -20°C.