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Pvu II
5´-CAG↓CTG-3´ 3´-GTC↑GAC-5´
Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
Enzyme: Pvu II
Product Size: 1500 units
Concentration: 15 U⁄µl
Star Activity: May exhibit star activity
Compatible Buffer: 10X Buffer M
Methylation Sensitivity: Not CpG methylation-sensitive
Optimal Reaction Temperature: 37° C
Sensitive to Heat Inactivation: No (Resistant to Heat Inactivation)
Shipping Condition: Approved for shipment on Wet or Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Storage Buffer
10 mM Tris-HCl, pH 7.5
100 mM KCl
0.1 mM EDTA
1 mM Dithiothreitol (DTT)
0.15% Triton® X-100
0.01% BSA
50% (v⁄v) glycerol
Loading Buffer
1% SDS
50% glycerol
0.05% Bromophenol blue
Store enzyme and buffer at -20°C, and loading buffer at RT. |