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Advanced DMEM/F-12
Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.
This Advanced DMEM/F-12 is manufactured as follows: With Without
• Glucose • L-glutamine
• Non Essential Amino Acids • HEPES
• Sodium pyruvate
• Phenol Red
The complete formulation is available.
Using Advanced DMEM/F-12
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX® I
Glutamine: No Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: Sodium Pyruvate
Serum Supplementation: Advanced (Reduced Serum)
Volume: 500 ml
Glucose: High Glucose
Product Size: 500 mL
Shipping Condition: Room Temperature
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture |