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TrypLE™ Express Enzyme (1X), phenol red
TrypLE™ Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.
Life Technologies offers a variety of TrypLE™ formulations for a range of cell culture applications.
Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Express is:
■Gentle on cells
■Room-temperature stable
■Easy to use
■Animal origin-free (AOF)
This TrypLE™ Express is modified as follows:
With
• Phenol Red
• EDTA
The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.
Gentle On Cells
TrypLE™ Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from enzymes present in some trypsin preparations and in other protease extracts.
Room-Temperature Stable
TrypLE™ Express is room-temperature stable and ready to use. TrypLE™ Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to Use
TrypLE™ Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Express, avoiding the need for trypsin inhibitors, such as FBS.
Chelators: EDTA
Phenol Red Indicator: Phenol Red
Classification: Animal Origin-Free
Enzyme Stability: Room Temperature Stable
Form: Liquid
Quantity: 1 universal bag(s)
pH Range: Check and Record
Osmolality: Check and Record
Concentrated: 1 X
Product Size: 5 L
Reagent Type: Trypsin Replacement
Tests Performed: In Vitro Bioassay
Green Features: Sustainable packaging,
Less hazardous,
Energy efficient
Shipping Condition: Room Temperature
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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