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Lumio™ Green In-Cell Detection Kit
Lumio™ Technology is designed for simple fluorescent detection of recombinant protein. The Lumio™ tag is a small (six amino acid) sequence that binds a fluorescent substrate, allowing you to visually detect protein localization in mammalian cells. The Mammalian Lumio™ Gateway® vectors (Figure 1) offer the following features:
■Lumio™ tag for accurate in vitro and in vivo protein detection
■CMV promoter for high-level constitutive expression
tR sites for efficient recombination with Gateway® entry clones
■Blasticidin resistance gene for fast, efficient selection
Use Mammalian Lumio™ Gateway® vectors for reliable and consistent protein expression, detection, and localization in mammalian cells (Figure 2). Vectors are available for generating protein with an N- and C-terminal Lumio™ fusion tag. In addition, each Mammalian Lumio™ Gateway® Kit includes a Lumio™ Green, Red, or Dual In-Cell Detection Kit. The Dual In-Cell Detection Kit is ideal for doing two color "pulse-chase" experiments to examine protein localization over time.
Color: Green
Platform: Electron Microscope,
Fluorescence Microscope
Label or Dye: Lumio™ Green
Product Size: 1 kit
Detection Method: Fluorescent
Detection Location: In-Cell Detection
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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