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Champion™ pET160 Gateway® Expression Kit with Lumio™ Technology
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.
Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, and reaction buffer.
Expression: E. coli
Promoter: T7
Bacterial or Yeast Strain: BL21 Star™(DE3),
TOP10
Cleavage: TEV Protease Recognition Site
Vector Type: pET
Product Size: 6 µg
Cloning Method: Gateway®
Inducing Agent: IPTG
Detection Location: In-Gel Detection
Expression Mechanism: Cell-Based Expression
Protein Tag or Fusion: Lumio,
His Tag (6x)
Selection Agent (Eukaryotic): None
Constitutive or Inducible System: Inducible
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
All destination vectors are provided lyophilized and supercoiled. |