Platinum® Blue PCR SuperMix
Platinum® Blue PCR SuperMix provides qualified reagents for the amplification of nucleic acid templates by PCR, and includes blue tracking dye for subsequent gel analysis. The reagents are premixed for high-throughput applications as well as general ease of use.
Using Platinum® Blue PCR SuperMix
The mixture contains recombinant Platinum® Taq DNA polymerase, Mg++, dNTPs, glycerol (used as a precipitant), and blue tracking dye. Platinum® Blue PCR SuperMix is supplied at a 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided sufficient for 100 or 1,000 amplification reactions of ~50 µL each.
Hot-start specificity
Specific binding of the anti-Taq antibody inhibits polymerase activity at room temperature. Activity is restored after a denaturation step in PCR cycling at 94°C, providing an automatic "hot start" in PCR and improving specificity and yield.
Polymerase: Platinum® Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: SuperMix or Master Mix
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Size: 1,000 reactions
Exonuclease Activity: 5' - 3'
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
|