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GB C-TERM GATEWAY IN VITRO KIT

产品编号: 12578035     查看说明书
产品名称: GB C-TERM GATEWAY IN VITRO KIT  .0   订购此产品 
供应商: Invitrogen
规格: 20 CLONING REACTIONS
目录价: 9600
库存状态: 三周到货
CAS编号:
应用范围: 生化试验
种属来源:
相关信息:

GeneBLAzer® C-terminal Gateway® Fusion Kit, for in vitro detection
The GeneBLAzer™ C-terminal Gateway® Fusion Kit contains the Gateway®-adapted destination
vector, pcDNA™6.2⁄cGeneBLAzer™-DEST, designed for use with the GeneBLAzer™ Technology. The
pcDNA™6.2⁄cGeneBLAzer™-DEST vector enables easy fusing of any gene to a mammalian-optimized b-lactamase gene, bla, and facilitates in vivo or in vitro detection of β-lactamase reporter activity in mammalian cells using a
unique fluorescent substrate. Use of the GeneBLAzer™ Technology provides a
highly sensitive and accurate method to quantitate gene expression in
mammalian cells. The vector contains the following elements:
• Human cytomegalovirus immediate-early (CMV) promoter⁄enhancer for
high-level expression in a wide range of mammalian cells
• β-lactamase bla(M) reporter gene for C-terminal fusion to the gene of interest
• Two recombination sites, attR1 and attR2, downstream of the CMV promoter
for recombinational cloning of the gene of interest from an entry clone
• Chloramphenicol resistance gene located between
Promoter: CMV 
Key Function: Reporter Assays 
Protein Tag or Fusion: BLA (Beta-Lactamase) 
Reporter: BLA (Beta-Lactamase)
Delivery Method: Transfection 
Cloning Method: Gateway® 
Selection Agent (Eukaryotic): Blasticidin 
Vector Type: GeneBLAzer Vectors 
Product Size: 1 kit 
Constitutive or Inducible System: Constitutive 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
 

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