GeneBLAzer® C-terminal Gateway® Fusion Kit, for in vitro detection
The GeneBLAzer™ C-terminal Gateway® Fusion Kit contains the Gateway®-adapted destination
vector, pcDNA™6.2⁄cGeneBLAzer™-DEST, designed for use with the GeneBLAzer™ Technology. The
pcDNA™6.2⁄cGeneBLAzer™-DEST vector enables easy fusing of any gene to a mammalian-optimized b-lactamase gene, bla, and facilitates in vivo or in vitro detection of β-lactamase reporter activity in mammalian cells using a
unique fluorescent substrate. Use of the GeneBLAzer™ Technology provides a
highly sensitive and accurate method to quantitate gene expression in
mammalian cells. The vector contains the following elements:
• Human cytomegalovirus immediate-early (CMV) promoter⁄enhancer for
high-level expression in a wide range of mammalian cells
• β-lactamase bla(M) reporter gene for C-terminal fusion to the gene of interest
• Two recombination sites, attR1 and attR2, downstream of the CMV promoter
for recombinational cloning of the gene of interest from an entry clone
• Chloramphenicol resistance gene located between
Promoter: CMV
Key Function: Reporter Assays
Protein Tag or Fusion: BLA (Beta-Lactamase)
Reporter: BLA (Beta-Lactamase)
Delivery Method: Transfection
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: GeneBLAzer Vectors
Product Size: 1 kit
Constitutive or Inducible System: Constitutive
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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