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SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity DNA Polymerase
The SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase offers the highest sensitivity available from Life Technologies for end point detection. This system combines the high thermostability of SuperScript® III RT with the specificity of Platinum® Taq DNA Polymerase High Fidelity to increase priming specificity, generate higher product yields, and identify a larger range of targets. The one-step format enables easy analysis of gene expression or detection of rare or viral RNA.
• Sensitivity - Routine detection down to 1 pg total RNA
• Convenience - Single-tube format for speed, convenience, and less reaction-to-reaction variability. Compatible with multiplexing
• Specificity - Uses SuperScript® III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
• Amplicon Size - Detection of targets from 300 bp to 10 kb for greater flexibility (Figure 1)
High-Fidelity PCR Amplification
Platinum® Taq DNA Polymerase
PCR Method: 1-Step RT-PCR
Reverse Transcriptase: SuperScript® III
Polymerase: Platinum® Taq DNA Polymerase High Fidelity
Hot Start: Built-In Hot Start
Sample Type (General): RNA
Product Size: 25 reactions
Detection Method: Gel Electrophoresis
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Final Product Size(s): 10 kb or less
Primer-Probe Compatibility: Unlabeled Oligos
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