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SuperScript® III One-Step RT-PCR System with Platinum®Taq
The SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase offers the highest sensitivity available for end point detection. This system combines the high thermostability of SuperScript® III RT with the specificity of Platinum® Taq DNA Polymerase to increase priming specificity, generate higher product yields, and identify a larger range of targets. The one-step format enables easy analysis of gene expression or detection of rare or viral RNA.
• Sensitive – Routine detection down to 0.01 pg total RNA (Figure 1)
• Convenience – One-step format for speed, convenience, and less reaction-to-reaction variability
• Specificity – Uses SuperScript® III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers (Figure 2)
• Amplicon Size – Compatible Detection of targets up to 4.5 kb in length for greater flexibility
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
PCR Method: 1-Step RT-PCR
Reverse Transcriptase: SuperScript® III
Polymerase: Platinum® Taq DNA Polymerase
Hot Start: Built-In Hot Start
Sample Type (General): RNA
Product Size: 25 reactions
Detection Method: Gel Electrophoresis
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Final Product Size(s): 4.5 kb or less
Primer-Probe Compatibility: Unlabeled Oligos
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