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BaculoDirect™ N-Term Transfection Kit
The BaculoDirect™ Baculovirus Expression System is a powerful and versatile eukaryotic system for high-level protein expression in insect cells. The combination of Gateway® Technology with baculovirus expression makes the BaculoDirect™ System the fastest and easiest method for production of recombinant baculovirus.
How it Works
The BaculoDirect™ Linear DNA (the baculovirus genome) is engineered to include attR sites for quick and efficient recombination with a Gateway® entry clone. The gene of interest is recombined from the entry clone into the BaculoDirect™ Linear DNA using a simple, one-hour LR reaction (Figure 1). The resulting reaction mix contains the recombinant baculovirus carrying the gene of interest and is used directly to transfect insect cells. The need for transforming bacteria and isolating a large bacmid or co-transfection of a transfer vector and linear baculovirus DNA into insect cells is eliminated. As a result, the hands-on time is greatly reduced. Purified
Cleavage: TEV Protease Recognition Site
Promoter: Polyhedrin
Vector Type: BaculoDirect Vectors
Product Size: 5 transfections
Cloning Method: Gateway®
Protein Tag or Fusion: V5 Epitope Tag,
His Tag (6x)
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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