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pIB/V5-His-DEST Vector
The pIB/V5-His-DEST vector is designed for rapid lambda phage site-specific recombination with a Gateway® entry clone and subsequent expression in Sf9, Sf21, or High Five™ insect cells. As part of the InsectSelect™ System, pIB/V5-His-DEST features:
■The OpIE2 promoter for constitutive expression
■Blasticidin resistance gene driven by the weak GP64 promoter for selection of stable cell lines that have integrated the plasmid into a transcriptionally active site
■C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and easy purification with a nickel-chelating resin
Promoter: OplE2
Vector Type: pDEST,
pIB
Product Size: 6 µg
Cloning Method: Gateway®
Protein Tag or Fusion: V5 Epitope Tag,
His Tag (6x)
Selection Agent (Eukaryotic): Blasticidin
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
The vectors are provided supercoiled and lyophilized and include 6 µg of expression vector and 10 µg of an expression control. The vectors are guaranteed stable for 6 months when stored properly. |