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Platinum® PCR SuperMix High Fidelity
Platinum® PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure). With Platinum® PCR SuperMix, High Fidelity, you'll:
• Achieve greater than six-times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 15 kb
• Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs
• Minimize PCR optimization
Using Platinum® PCR SuperMix, High Fidelity
Platinum® PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic "hot-start" PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
Polymerase: Platinum® Taq DNA Polymerase High Fidelity
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 6 X
GC-Rich PCR Performance: Low
Reaction Format: SuperMix or Master Mix
Product Overhang: Mixed
Reaction Speed: Standard
Final Product Size(s): 15 kb or less
Product Size: 5,000 reactions
Exonuclease Activity: 5' - 3'
Shipping Condition: Approved for shipment on Wet or Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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