ADVANCED MEM

Advanced MEM 
Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2.
This Advanced MEM is manufactured as follows:
With Without
• Glucose • L-glutamine
• Non Essential Amino Acids • HEPES
• Sodium pyruvate 
• Phenol Red 
The complete formulation is available.
Gibco® Advanced MEM is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX® I lipid-rich bovine serum albumin for cell culture, and the trace
Glutamine: No Glutamine 
Phenol Red Indicator: Phenol Red 
Form: Liquid 
HEPES Buffer: HEPES 
Sodium Pyruvate Additive: Sodium Pyruvate 
Volume: 500 ml
Glucose: Low Glucose 
Product Size: 10 x 500 mL 
Serum Supplementation: Advanced (Reduced Serum) 
Green Features: Sustainable packaging 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.