ADVANCED DMEM

Advanced DMEM 
Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.
This Advanced DMEM is manufactured as follows: With Without
• High glucose • L-glutamine
• Non Essential Amino Acids 
• Sodium pyruvate 
• Phenol Red 
The complete formulation is available.
Using Advanced DMEM
Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX® I lipid-rich bovine serum albumin for
Glutamine: No Glutamine 
Phenol Red Indicator: Phenol Red 
Form: Liquid 
Glucose: High Glucose 
Serum Supplementation: Advanced (Reduced Serum) 
HEPES Buffer: No HEPES 
Sodium Pyruvate Additive: Sodium Pyruvate 
Volume: 500 ml 
Product Size: 10 x 500 mL 
Green Features: Sustainable packaging 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.