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One Shot® MAX Efficiency™ DH10B™ T1 Phage-Resistant Cells
DH10B™ T1 Phage-Resistant E. coli have all the benefits of the DH10B™ genotype, with the addition of the tonA genotype which confers resistance to T1 and T5 phage infection. The inclusion of the tonA genotype protects your valuable samples and minimizes the possibility of downtime in your lab due to phage contamination. The DH10B™-T1R genotype has the following features:
■tonA genotype to confer resistance to T1 and T5 phage
■Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries (1,2)
■The endA1 mutation to increase plasmid yield and quantity
ZΔM15 for blue/white screening of recombinant clones
DH10B™-T1R E. coli are available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High efficiency transformation is seen with plasmids 150 kb in size (3).
Competent Cell Type: Chemically Competent
Species: E. coli
Format: One Shot
Transformation Efficiency: > 1x10^9
Bacterial or Yeast Strain: DH10B™
Product Size: 20 reactions
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: Yes
Cloning Methylated DNA: Yes
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Lacks F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): Yes
High Throughput Compatibility: Not High Throughput-Compatible
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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