ThermoScript™ Reverse Transcriptase ThermoScript™ Reverse Transcriptase (RT) is an avian RT with reduced RNase H activity designed for RT-PCR (1). It is engineered to have high thermal stability (up to 70°C) (Figure 1), copy RNA with secondary structure, and improve priming specificity (2). • Yield – Reduced RNase H activity results in greater first-strand cDNA yields • Size of cDNA – Generation of RT-PCR products up to 12 kb • Thermostability – ThermoScript™ Reverse Transcriptase is active up to 70°C • Flexibility – Uses either total or poly(A)+ RNA Unit Definition: One unit of Platinum® Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min. at 74°C. For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Optimal Reaction Temperature: 65° C Final Product Size(s): 12.3 kb or less Reverse Transcriptase: ThermoScript™ Ribonuclease H Activity: Reduced Form: Frozen Format: Tube(s) Quantity: 375 units Template: ssRNA Sensitivity: Medium Product Size: 375 units Concentration: 15 U&⁄µl Enzyme Function: RNA dependent DNA Polymerase Shipping Condition: Dry Ice Number of Reactions: 25 Reactions Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.