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PureLink® RNA Micro Scale Kit
The PureLink® RNA Micro Scale Kit provides rapid purification and concentration of total RNA from a variety of samples (e.g., LCM tissues, fine needle aspirates, and FACS-sorted cells). RNA purification using the PureLink® RNA Micro Scale Kit is:
• Effective—expect excellent RNA recovery (up to 100 µg total RNA) from small samples
• Convenient and safe—the extraction procedure uses no phenol/chloroform, CsCl gradients, or precipitation with either LiCl or ethanol
• Fast—obtain high-quality total RNA, typically in just 15 minutes
• Reliable—obtain ready-to-use RNA suitable for any downstream application
A simple and reliable technology for everyday RNA purification
PureLink® technology combines guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can be completed in typically less than 15 minutes with >85% recovery from the first elution. The kit can be used for clean-up of purified RNA
Sample Type (General): Bacteria,
Yeast,
Cells,
Plant Samples,
Tissue,
Liquid Samples (e.g. Serum)
High Throughput Compatibility: Not High Throughput-Compatible
Downstream Application: RT-PCR (Endpoint),
cDNA Library Construction,
Nuclease Protection Assays,
Nucleic Acid Labeling,
Real-Time PCR,
Northern Blotting
Scale: Micro
Format: Spin Column
System: PureLink™
Product Size: 50 preps
Final Product: Total RNA
Yield (up to): 100 µg
Purification Time: 15 min
Elution Volume - max: 10 µl
Elution Volume - min: 5 µl
Isolation Technology: Silica Spin Column
Sample Type (Specific): Yeast,
Bacteria,
Plant,
Tissue (Mammalian),
Mammalian Cells,
Fine Needle Aspirates (FNA),
Laser Capture Microdissection (LCM)
Purity or Quality Grade: Molecular Biology Grade
Starting Material (Amount): Less than 10 mgs tissue,
Up to 5 x 10^5 cells
Shipping Condition: Room Temperature
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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