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PureLink® Pro 96 total RNA Purification Kit
The PureLink® Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples (Figures 1 and 2).
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.
Improved plate design
The PureLink® Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
Sample Type (General): Bacteria,
Yeast,
Cells,
Plant Samples,
Tissue,
Blood
High Throughput Compatibility: High Throughput-Compatible
Downstream Application: RT-PCR (Endpoint),
Real-Time PCR,
Nuclease Protection Assays,
Northern Blotting
Scale: Mini
Format: 96-well plate
System: PureLink™
Platform: Robotic Liquid Handling Systems,
Vacuum Manifold,
TECAN Freedom Evo,
Centrifuge,
TECAN Genesis,
Eppendorf epMotion,
Biomek® FX
Quantity: 1 kit
Product Size: 4 plates
Final Product: Total RNA
Yield (up to): 33 µg
Purification Time: 20 min
Number of Reactions: 96 Preps
Elution Volume - max: 150 µl
Elution Volume - min: 50 µl (centrifuge),
100 µl (vacuum)
Isolation Technology: Silica Plate
Sample Type (Specific): Blood,
Yeast,
Mammalian Cells,
Cell Cultures,
Plant,
Tissue (Mammalian),
Bacteria,
Insect Cells
Purity or Quality Grade: Molecular Biology Grade
Shipping Condition: Room Temperature
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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