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ElectroMAX™ DH10B™ T1 Phage-Resistant Competent Cells
ElectroMAX™ DH10B™ T1 Phage-Resistant electrocompetent E. coli cells offer transformation efficiencies that are among the highest achieved by any product offered by Life Technologies, and can exceed 1 x 1010 cfu/µg plasmid DNA in efficiency. They are ideal for applications requiring high efficiency transformation, including cDNA and gDNA library construction.
• Maximizes yield of transformants from limited cloning products
• Permits efficient cloning of methylated DNA
• Resistant to T1 and T5 bacteriophages
• Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Delivers high yield plasmid preparations for downstream applications
Phage-Resistant Representative Libraries from a Single Transformation
The tonA mutation in DH10B™ confers resistance to bacteriophages T1 and T5 (1), safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA, mcr
Competent Cell Type: Electrocompetent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^10
Bacterial or Yeast Strain: DH10B™
Plasmid Type: May be used for plasmids > 20 kb
Product Size: 25 reactions
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: Yes
Cloning Methylated DNA: Yes
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Lacks F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): Yes
High Throughput Compatibility: Not High Throughput-Compatible
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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