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SuperScript® One-Step RT-PCR System for Long Templates
The SuperScript® One-Step RT-PCR for Long Templates is designed for the convenient, sensitive, and reproducible detection and analysis of RNA molecules by one-step RT-PCR. Both cDNA synthesis and PCR are performed in a single tube using gene-specific primers and specific target RNAs from either total RNA or mRNA. Due to the high fidelity of the DNA polymerase blend in the enzyme mix, this kit can also be used for one-step cloning of genes.
Using the SuperScript® One-Step System
The system consists of two major components: SuperScript® II RT/Platinum® Taq Hi Fi Mix and 2X Reaction Mix. The enzyme mix is a mixture of SuperScript® II Reverse Transcriptase, which provides reduced RNase H activity and increased thermal stability, and Platinum® Taq DNA Polymerase High Fidelity, which is a blend of recombinant Taq DNA polymerase and the proofreading enzyme Pyrococcus species GB-D thermostable polymerase. The Taq DNA polymerase in the blend is complexed with a
PCR Method: 1-Step RT-PCR
Reverse Transcriptase: SuperScript® II
Polymerase: Platinum® Taq DNA Polymerase High Fidelity
Hot Start: Built-In Hot Start
Sample Type (General): RNA
Product Size: 25 reactions
Detection Method: Gel Electrophoresis
Shipping Condition: Wet Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Final Product Size(s): 9 kb or less
Primer-Probe Compatibility: Unlabeled Oligos
SuperScript® One-Step RT-PCR System components are listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored. |