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Description
SuperScript First-Strand Synthesis System for RT-PCR is optimized for sensitive RT-PCR from low-input RNA, <50 ng. It is suitable for generating first-strand cDNA from total or poly(A) + RNA (1). In conjunction with PCR, the system can be used to detect the presence of rare message, to quantitate the amount of specific mRNA from small numbers of cells, or to clone specific cDNAs without constructing an entire cDNA library. The system is flexible, allowing the use of any PCR enzyme. Combine with AccuPrime™ Taq or Platinum® Taq DNA Polymerase for higher specificity PCR or with AccuPrime™ Pfx DNA Polymerase for high-fidelity cloning applications.
The SuperScript® First-Strand Synthesis System for RT-PCR:
Is optimized for RT-PCR
Uses SuperScript® II RT for greater first-strand cDNA yields
Detects as little as 50 to 100 molecules of RNA template from only 1 pg of total RNA (Figure 1)
Includes oligo(dT) and random hexamers to prime cDNA synthesis for flexible use
Amplifies first-strand cDNA products directly without organic extractions or precipitations |