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SuperScript® VILO™ cDNA Synthesis Kit
The SuperScript® VILO™ cDNA Synthesis Kit provides the high-temperature capability of SuperScript® III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR). VILO is an acronym for variable input, linear output; and the SuperScript®: VILO™ cDNA Synthesis Kit provides the most consistent results for your RT experiments, with unprecedented linearity across the broadest range of input material.
Using the SuperScript® VILO™ cDNA Synthesis Kit
This formulation provides enhanced cDNA synthesis efficiency and can be used with very low and very high amounts of input RNA (up to 2.5 µg total RNA in a 20 µL reaction), giving a linear response in message abundance as measured by qPCR. The 10X SuperScript® Enzyme Mix includes SuperScript® III RT, RNaseOUT™ Recombinant Ribonuclease Inhibitor, and a proprietary helper protein.
SuperScript® III RT
SuperScript® III RT is an engineered version of M-MLV RT with reduced RNase
Reverse Transcriptase: SuperScript® III
Downstream Application: Real-Time PCR
Reaction Format: SuperMix or Master Mix
Sample Type (General): RNA
Primer Type: Random Primers
Product Size: 250 x 20 µL reactions
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Optimal Reaction Temperature: 42° C
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