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CloneChecker™ System (for screening bacterial colonies)
CloneChecker™ System is a rapid method for screening recombinant bacterial colonies or liquid cultures of colonies for the presence of target plasmid DNA (1). The CloneChecker™ System:
■Screens bacterial colonies for plasmid DNA and insert of interest prior to purification
■Screens for inserts >200 bp in plasmids up to 15 kb by size comparison to the original vector or a DNA size marker
■Goes from plated colonies through lysis in less than 5 minutes
■Allows for supercoiled, restriction digest, or PCR analysis
■Works with single-copy plasmids starting with a 2-mm bacterial colony
■Includes sufficient reagents for processing 100 bacterial samples by supercoiled analysis (direct size comparison); and 100 samples by restriction endonuclease or PCR analysis
High Throughput Compatibility: Manual Protocols,
Not High Throughput-Compatible
Sample Type (General): Bacteria
Downstream Application: PCR,
Cloning
Product Size: 100 preps
Final Product: Plasmid DNA
Sample Type (Specific): Bacterial Culture
Shipping Condition: Room Temperature
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
The CloneChecker™ System includes Yellow, Red, and Green solutions and Loading buffer. Store at 15°C to 30°C. |