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PureLink® HiPure Plasmid Filter Midi/Maxi Starter Kit
PureLink® HiPure Filter Plasmid Kits are designed to isolate plasmid DNA of the highest purity from 25 to 500 mls of bacterial cultures (Table 1). The PureLink® HiPure Plasmid Filter Kits include HiPure Filter Columns, which combine filtration for clarification of the bacterial lysate with anion-exchange resin purification of plasmid DNA, in a single unit. The resulting plasmid DNA is of a quality equivalent to that obtained by purification with two passes through cesium chloride gradients - the most rigorous method for DNA purification. Purify high quality DNA in less than two hours for transfections and other applications with no need for additional steps to remove contaminants like RNA, proteins, or endotoxins. Additionally, the elimination of phenol, chloroform, ethidium bromide, and cesium chloride from the protocol minimizes exposure to, and disposal of hazardous materials.
The PureLink® HiPure Plasmid Filter Kits provide:
• Simple protocols - bacterial lysate clarification
High Throughput Compatibility: Manual Protocols,
Not High Throughput-Compatible
Sample Type (General): Bacteria
Downstream Application: Transfection,
Cloning,
Next-Generation Sequencing,
In Vitro Transcription,
Sequencing,
Nucleic Acid Labeling,
Transformation,
PCR
System: PureLink™
Quantity: 4 columns
Plasmid Type: High Copy Plasmid,
Low Copy Plasmid,
BAC,
<40kb
Product Size: 2 preps
Final Product: Plasmid DNA
Yield (up to): 1000 µg
Purification Time: 90 min
Number of Reactions: 4 Preps
Elution Volume - max: 15 ml
Elution Volume - min: 15 ml
Isolation Technology: Anion Exchange Resin with Lysate Clearing Filter
Sample Type (Specific): Bacterial Culture
Purity or Quality Grade: Transfection Grade
Shipping Condition: Room Temperature
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Store at room temperature. |