Platinum® GenoType Tsp DNA Polymerase Platinum® GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic "hot-start" method that improves PCR specificity and allows for room temperature reaction assembly. Platinum® GenoType Tsp DNA Polymerase: • Exhibits minimal non-templated nucleotide addition to PCR products • Lacks both 5´ and 3´ exonuclease activity • Amplifies fragments up to 500 bp Application Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirable. Unit definition One unit of Platinum® GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standard Taq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C under optimized reaction conditions. Polymerase: Tsp Genotyping Polymerase Hot Start: Built-In Hot Start Fidelity (vs. Taq): 1 X GC-Rich PCR Performance: Low Reaction Format: Separate Components Product Overhang: Blunt Reaction Speed: Standard Final Product Size(s): 500 bp or less Product Size: 2500 units Exonuclease Activity: None Shipping Condition: Approved for shipment on Wet or Dry Ice Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures