ThermoScript™ RT-PCR System & Platinum® Taq DNA Polymerase High Fidelity The ThermoScript™ RT-PCR System is designed for the detection and analysis of RNA in a two-step process. ThermoScript™ reverse transcriptase (RT) is used to generate full-length cDNA transcripts using either total RNA or poly(A)+ RNA. PCR amplification is then performed using Platinum® Taq DNA Polymerase High Fidelity. • cDNA Yield—Low RNase H activity of ThermoScript™ RT maximizes first-strand cDNA yield • Thermostability—ThermoScript™ RT is active at temperatures up to 70°C • Fidelity—Platinum® Taq DNA Polymerase High Fidelity offers 6 times the fidelity of Taq DNA Polymerase alone • Amplicon Size—Platinum® Taq DNA Polymerase High Fidelity is ideal for templates from 100 bp to 15 kb (Figure 1) High-Yield First Strand Synthesis ThermoScript™ Reverse Transcriptase (RT) is an avian RT with reduced RNase H activity designed for RT-PCR of your most highly structured, difficult RNA templates. ThermoScript™ RT-PCR Systems are designed to read through PCR Method: 2-Step RT-PCR Reverse Transcriptase: ThermoScript™ Polymerase: Platinum® Taq DNA Polymerase High Fidelity Hot Start: Built-In Hot Start Sample Type (General): RNA Product Size: 100 reactions Detection Method: Gel Electrophoresis Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures. Final Product Size(s): 12 kb or less Primer-Probe Compatibility: Unlabeled Oligos