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SuperScript® One-Step RT-PCR System with Platinum® Taq DNA Polymerase
The SuperScript® One-Step RT-PCR System with Platinum® Taq DNA Polymerase is designed for the convenient, sensitive, and reproducible detection and analysis of RNA molecules by RT-PCR. Components for both cDNA synthesis and PCR are combined in a single tube, using gene-specific primers and target RNAs from either total RNA or mRNA. Reverse transcription automatically follows PCR cycling without additional steps.
Using the SuperScript® One-Step System
The system consists of two major components: RT/ Platinum® Taq Mix and 2X Reaction Mix. The RT/ Platinum® Taq Mix contains a mixture of SuperScript® II Reverse Transcriptase and Platinum® Taq DNA Polymerase for optimal cDNA synthesis and PCR amplification. SuperScript® II RT is a modified version of Moloney Murine Leukemia Virus (M-MLV) RT, engineered to reduce RNase H activity and increase thermal stability. Platinum® Taq DNA polymerase is Taq DNA polymerase complexed with a proprietary antibody that inhibits
PCR Method: 1-Step RT-PCR
Reverse Transcriptase: SuperScript® II
Polymerase: Platinum® Taq DNA Polymerase
Hot Start: Built-In Hot Start
Sample Type (General): RNA
Product Size: 25 reactions
Detection Method: Gel Electrophoresis
Shipping Condition: Wet Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Final Product Size(s): 3 kb or less
Primer-Probe Compatibility: Unlabeled Oligos
SuperScript® One-Step RT-PCR System components are listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored. |