Citrate Buffer (pH 6.0), Ready-To-Use Citrate buffer is used in Heat Induced Epitope Retrieval (HIER) methods. HIER is used to reverse the loss of antigenicity that occurs with some epitopes in formalin-fixed paraffin embedded tissues. During epitope retrieval, tissue slides are immersed in a solution and heated (>95° C) by hot plate or other appropriate heat source such as a pressure cooker or steamer. Citrate buffer is the preferred solution for most antibodies. Reagent Type: Antigen Retrieval Reagent Product Size: 1 L Shipping Condition: Wet Ice Regulatory Statement: For Laboratory Use Store in refrigerator (2–8°C).