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现货|Mueller Hinton Broth|MH肉汤
原理：
Acid hydrolysate (digest) of casein and beef extract supply amino acids and other nitrogenoussub stances,minerals, vitamins, carbon and other nutrients to support the growth of microorgan-isms. Starch acts as a protective colloid against toxic substances that may be present in the medium. Hydrolysis of the starch during autoclaving provides a small amount of dextrose, which is a source of energy.
Directions for Preparation from Dehydrated Product
1. Suspend the powder in 1 L of purified water:
Difco Mueller Hinton Broth – 21 g;
BBL Mueller Hinton Broth – 22 g.
Mix thoroughly.
2. Heat with frequent agitation and boil for 1 minute to com-
pletely dissolve the powder.
3. Autoclave at 116-121°C for 10-15 minutes (consult product
label). DO NOT OVERHEAT.
4. Check prepared medium to ensure the final pH is 7.3 ± 0.1
at 25°C.
5. Test samples of the finished product for performance using
stable, typical control cultures
过程：
For a complete discussion on broth dilution antimicrobial
susceptibility testing, refer to the appropriate procedures
outlined in the references.
Organisms to be subcultured must first be isolated in pure
culture on an appropriate solid medium. Transfer growth from
the isolation medium to Mueller Hinton Broth using standard
bacteriologic techniques.
For enrichment purposes, inoculate the specimen onto primary
media and then into the broth, according to recommended
procedures.
Incubate the tubes at 35°C under conditions appropriate for the organism being cultured.
预期结果：
For broth dilution antimicrobial susceptibility testing, refer to appropriate references for results.Growth in broth media is indicated by the presence of turbidity compared with an uninoculated control.
用途：
Mueller Hinton Broth is a general purpose medium that may be
used in the cultivation of a wide variety of fastidious and non-fastidious microorganisms. This medium is not supplemented
with calcium or magnesium ions. |