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现货|Brilliant Green Bile 2%|2%亮绿胆盐肉汤
Intended Use
Brilliant Green Bile Broth 2% (Brilliant Green Lactose Bile Broth)
is used for the detection of coliform organisms in foods, dairy
products, water and wastewater, as well as in other materials
of sanitary importance.
Principles of the Procedure
Brilliant Green Bile Broth 2% contains two inhibitors of both gram-positive and selected gram-negative organisms; i.e., oxgall and brilliant green dye. Organisms, primarily coliforms, which are resistant to the action of the inhibitors and which ferment the lactose, are able to replicate in this medium. Fermentation is detected by gas production.
Directions for Preparation from Dehydrated Product
1.Suspend 40 g of the powder in 1 L of purified water. Mix horoughly.
2.Warm slightly to completely dissolve the powder.
3.Dispense into tubes containing inverted fermentation vials.
4.Autoclave at 121°C for 15 minutes. Cool the broth as quickly as possible.
5.Test samples of the finished product for performance using stable, typical control cultures.
Procedure
For the detailed procedures for use of this medium in confirma-tory testing for coliforms, refer to the various compendia for the examination of materials of sanitary importance. |