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Brain Heart Infusion Agar|脑心琼脂|现货进口
原理：
BHI Agar derives its nutrients from the brain heart infusion, peptone and dextrose components. The peptones and infusion are sources of organic nitrogen, carbon, sulfur, vitamins and trace substances. Dextrose is a carbohydrate source that microorganisms utilize by fermentative action. The medium is buffered through the use of disodium phosphate.When defibrinated sheep blood is added to the basal medium, it provides essential growth factors for the more fastidious fungal organisms.
过程：
Prepare plated medium from tubed agar deeps by liquefying the medium in boiling water, cooling to 45-50°C and pour-ing into sterile Petri dishes. Additives (e.g., blood) can be used as desired.
Use standard procedures to obtain isolated colonies from specimens. Since many pathogens require carbon dioxide on primary isolation, plates of plain BHI may be incubated in an atmosphere containing approximately 5-10% CO2. Incubate plates at 35 ± 2°C for 24-48 hours.
For isolation of fungi from potentially contaminated specimens, a selective medium should be inoculated along with the nonse-lective medium. Incubate the plates at 25-30°C in an inverted position (agar side up) with increased humidity. For isolation of fungi causing systemic mycoses, two sets of media should be inoculated, with one set incubated at 25-30°C and a duplicate
set at 35 ± 2°C. All cultures should be examined at least weekly for fungal growth and should be held for 4-6 weeks before being reported as negative.
BHI Agar slants primarily are used for the cultivation and main-tenance of pure cultures of microorganisms.
用途：
Brain Heart Infusion (BHI) Agar is a general-purpose medium suitable for the cultivation of a wide variety of organism types, including bacteria, yeasts and molds. With the addition of 5% or 10% sheep blood, it is used for the isolation and cultivation of a wide variety of fungal species, including systemic fungi,
from clinical and nonclinical sources. |