|
FlowCellect™ Human ESC Nucleus Marker Characterization Kit
Close
PRODUCT FAMILY INFORMATION
Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
» More…
Description:
FlowCellect™ Human ESC Nucleus Marker Characterization Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s The FlowCellect™ Human Embryonic Stem Cell Characterization kit is designed as an easier and quicker way to track surface marker expression of hOCT4, SSEA4 and SSEA1. The kit will also help to determine the percentage of undifferentiated human embryonic stem cells in culture by determining the percentage of cells that express both hOCT4 and SSEA4 and not SSEA1. This quick test will allow researchers to determine the quality of their cells in culture as well as see changes in marker expression during a differentiation protocol.
Sufficient reagents are provided for 25 3-color samples and 5 isotype control tests. The kit includes all optimized fluorescently labeled antibodies and buffers necessary for analysis. To verify antibody specificity, all antibodies were tested against the ENSTEM-A cell line that does not express hOCT4, SSEA4 or SSEA1. A cell strainer is also included to ensure samples are devoid of large cell clumps that can alter cell analysis. Detailed assay instructions are also included to assist in analysis and to ensure the correct cell concentration is obtained for flow cytometric analysis.
View All »
Background Information:
Human embryonic stem cells (hESCs) are unique cells in that they can be maintained in vitro as pluripotent cells and can be induced to differentiate into many cell types by manipulating culture conditions. Therefore, hESCs have the potential of providing an unlimited supply of cells for cell-based therapy as well as providing a resource to study basic human developmental biology. Subsequently, much attention has been focused on directing hESC differentiation along specific developmental lineages [1-4].
Currently, the most utilized method to test for the undifferentiated state of hESCs is to analyze the expression of the proteins OCT4, NANOG, Alkaline phosphatase, SSEA3, SSEA4, TRA-1-60, and TRA-1-81 [5]. The transcription factor, OCT4 is essential for the formation and maintenance of pluripotent embryonic stem cells (6,7). High levels of OCT4 expression is seen in undifferentiated hESCs and is downregulated upon differentiation (8), thus making OCT4 an important marker to study hESCs. The stage specific embryonic antigens or SSEAs (SSEA1, 3 and 4) are also good markers of undifferentiated hESCs (9). The antibodies for SSEAs recognize epitopes on related glycosphingolipids or GSLs (10). The SSEA4 antibody recognizes the terminal sialic acid on GSL-7 (10). Upon differentiation, SSEA4 expression levels decrease and SSEA1 expre |