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FlowCellect™ Multi-STAT Activation Profiling Kit
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PRODUCT FAMILY INFORMATION
Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
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Description:
FlowCellect™ Multi-STAT Activation Profiling Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ Multi-STAT Activation Kit is designed to simultaneously detect the phosphorylation of the most commonly studied STAT proteins through cytokine or growth factor mediated pathway activation. The kit will also allow a researcher to quickly profile the status of constitutive activation of STAT1, STAT3, and STAT5 within a population of cells or primary tumors.
The ability to detect the activation of multiple STAT proteins simultaneously allows researchers to determine and profile the STAT signaling pathways within a cell type of interest. Alterations in these signaling profiles can be used to monitor the effect of a particular upstream treatment targeting the STAT pathways.
Antibodies against phospho-STAT1, 3, and 5A/B are provided in the kit to monitor the activation state of each STAT within a given cell type. U937 cells were stimulated with Gamma Interferon, IL-6, and GM-CSF simultaneously, to activate STAT1, STAT3, and STAT5A/B, respectively. When performing a three color stain, triple positive cell populations were detected.
Sufficient reagents are provided for 25 3-color samples. The kit includes all optimized fluorescently labeled antibodies and buffers necessary for cell preparation and analysis. Detailed assay instructions are included to assist in analysis and to ensure the correct cell concentration is obtained during acquisition of sample data.
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Background Information:
STATs are transcription factors that can be phosphorylated normally by a cell surface receptor such as cytokine receptors, followed by dimerization and nuclear translocation where they bind to DNA and activate transcription of their target genes. STATs are involved in embryogenesis, cellular immunity, and cell proliferation [S Akira (1999)].
Many types of cancers both solid tumor and some blood based malignancies have been found to exhibit constitutive STAT activation [Ralf Buettner (2002)]. Interestingly, the activation within these cancer cells is generally a combination of STATs, typically STAT1, STAT3, and STAT5. This has led to an increase in research revolving around the complex family of STATs.
Oncogenesis and tumour progression are supported by alterations in cell signaling. Using flow cytometry, it is now possible to track and analyze signaling events in individual cancer cells [Jonathan M. Irish (2006)]. Single cell resolution of signaling molecules allows for a greater understanding of the elaborate processes that occur when cells transform and begin |