|
FlowCellect™ PI3K/MAPK Dual Pathway Activation and Cancer Marker Detection kit
Close
PRODUCT FAMILY INFORMATION
Phospho-Akt Antibodies, Kits & Assays
Millipore’s phosphoAkt antibodies have been well validated and published. See below or a comprehensive list of our phosphoAkt products, based on the expertise of Upstate & Chemicon.
EMD Millipore’s phosphoAkt antibodies are based on the expertise of Upstate & Chemicon. Our phosphoAkt antibodies have been well validated and published. See the ordering tab for a comprehensive list of our phosphoAkt products and direct links to their technical pages for data, references and related products.
» More…
MAPK Antibodies, Proteins, Inhibitors, & Assays
Offering hundreds of premium quality antibodies, enzymes, activity assays, and small molecule inhibitors for MAPK signaling research
EMD Millipore offers hundreds of premium quality antibodies, enzymes, activity assays, and small molecule inhibitors for MAPK signaling research. All EMD Millipore products are based on the expertise of Upstate, Chemicon, and Calbiochem.
» More…
Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
» More…
Description:
FlowCellect™ PI3K/MAPK Dual Pathway Activation and Cancer Marker Detection kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Recent evidence suggests that cross-talk between the PI3K and MAPK signaling pathways exist. We use pAkt and pERK antibodies to examine the PI3K/MAPK interactions. Additionally, to further interrogate the interplay between these two pathways, cell proliferative marker Ki-67 is used to validate the final biological effect.
Millipore’s FlowCellect™ PI3K/MAPK Dual Activation and Cancer Detection Kit is designed to examine this cross-talk in a multi-parametric fashion by providing three fully validated and optimized antibody biomarkers to measure specific cell signaling events in flow applications. The three antibodies provided in the kit are Anti-phospho-Akt Alexa Fluor 488 conjugate, Anti-phospho-ERK R-Phycoerythrin conjugate, and Anti-Ki-67 PerCP conjugate. By utilizing all three antibody biomarkers simultaneously in flow applications, we now have the ability to thoroughly evaluate the “cross-talk” between PI3K and MAPK pathways and to further determine the consequence of their interplay in cell proliferation and differentiation by measuring their effect on Ki-67 expression.
Phosphorylated Akt and phosphorylated ERK are included in the kit to provide the end user with the means to cross-examine both the PI3K and MAPK signaling pathways simultaneously. It has been suggested that the phosphorylation of Akt can result in the inhibition, or dephosphorylation, of phospho-Raf on Ser 259 [Jun, T. |