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FlowCellect Chemokine Receptor CCR2B Surface Expression Identification and Quantification Kit
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PRODUCT FAMILY INFORMATION
Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
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Description:
FlowCellect Chemokine Receptor CCR2B Surface Expression Identification and Quantification Kit
Trade Name:
FlowCellect
Qty/Pk:
100 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ GPCR Surface Identification kit is designed to provide an easier and more efficient way of characterizing GPCR expression on a given cell type of interest. This kit includes optimized buffers and a high affinity, specific antibody capable for detecting the GPCR target in question. Also included in the kit is a secondary antibody conjugate to R-Phycoerythrin, which is a bright fluorochrome ideal for measuring endogenous levels of GPCR expression on native cells. By following the steps outlined in the assay procedure, researchers can easily identify which native cell lines would be suitable for performing secondary assays in support of their structure-activity drug discovery efforts.
Additional test applications include the identification of high, medium, and low expressing cell cultures during the clonal selection process. When a researcher decides to build a stable cell line in-house, it is essential that the clone of choice will provide the GPCR surface expression needed to perform drug screening. Choosing the wrong clone can adversely affect a primary screen if the chosen clone cannot detect lower affinity compounds. This can lead to a lower hit rate, which can result in screening limitations when identifying potential blockbuster compounds.
Another added feature is the ability to quantitatively measure GPCR cell surface expression using flow cytometry as the method for detection. Provided in the kit are two vials of Millipore’s ChemiScreen™ GPCR cell lines, serving as assay controls. Using patented technology, Millipore’s ChemiScreen™ GPCR cell lines retain high surface expression as illustrated by the relatively high maximal number of binding sites (Bmax value) when compared to a native system.
By using Millipore’s FlowCellect™ Chemokine Receptor Surface Expression Identification kit, the researcher has the ability to identify a native GPCR expressing cell for biological assay development; the researcher can also easily select clonal stable cell lines for these GPCRs; finally, the researcher can monitor the changes of these receptor expression levels during a disease state.
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Background Information:
The FlowCellect™ GPCR identification kits are designed for flow cytometry applications, which can be used for the identification and quantification of the surface expression level of GPCRs on any cells. The kit uses a GPCR antib |