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FlowCellect™ Cell Cycle Checkpoint ATM DNA Damage Kit
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PRODUCT FAMILY INFORMATION
Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
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Description:
FlowCellect™ Cell Cycle Checkpoint ATM DNA Damage Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ DNA Damage (ATM) and Cell Cycle Analysis Kit is designed to identify the linkage between DNA damage in relation to cell cycle checkpoints in flow cytometry applications. The kit includes a directly conjugated Anti-phospho-ATM (Ser1981) Alexa Fluor® 488 conjugate plus a DNA dye (Propidium Iodide) which allows the researcher to perform bivariate analysis to identify where DNA damage takes place relative to the positioning within the cell cycle. The kit includes an optimized protocol with all of the necessary components to provide the researcher a true “plug and play” design for their research when studying DNA damage and cell cycle checkpoints. This immunocytochemical method is very sensitive, and it offers an assay to screen anti-tumor drugs at every phase of the cell cycle.
In all, a comprehensive understanding of DNA damage and its association with cell cycle behavior can provide the researcher with useful information which will be important in interpreting the intrinsic nature of cell proliferation, apoptosis, DNA repair, and assist in the development of anti-neoplastic agents. Millipore’s FlowCellect™ DNA Damage (ATM) and Cell Cycle Analysis Kit is designed to allow the researcher to easily identify, discriminate, and quantitate cells undergoing DNA damage relative to its positioning within the cell cycle.
All FlowCellect kits are optimized on guava® bench top flow cytometers. FlowCellect kits can be used on any flow cytometer following the same protocol providing researchers a reliable and fully validated solution to study DSBs and cell cycle distribution right in the comfort of their own lab. This kit contains optimized fixation, permeabilization, wash, and flow buffers to provide researchers with a complete solution for bivariate analysis to assess DNA damage in relation to positioning within the cell cycle.
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Background Information:
Ataxia telangiectasia mutated (ATM) is a protein kinase that becomes activated in response to DNA damage, particularly when the damage involves formation of DNA double-strand breaks (DSBs). Detection of DNA damage due to DSBs is a fundamental method for assessing cell health and evaluation of anti-neoplastic agents. Bivariate analysis monitoring the extent of ATM phosphorylation along with a DNA dye offers a sensitive and valuable tool in studying the factors that induce DNA damage (measured by the level of DSBs) and/or affect DNA repair. Understanding where the first indicatio |