|
Human Plasma Fibronectin Purified Protein
Close
PRODUCT FAMILY INFORMATION
Fibronectin Antibodies, Proteins & Assays
Millipore’s Fibronectin Antibodies, proteins, and assays are based on the expertise of Upstate & Chemicon. See below products related to Fibronectin research, including Migration assays & ELISA kit.
EMD Millipore’s Fibronectin related Antibodies, proteins and assays are based on the expertise of Upstate & Chemicon. All have been well validated and published. See below for data, references and related products for Fibronectin.
ECM Proteins and Coated Plates
Meet cell type-specific needs for growth and adhesion
Millipore offers a wide variety of ECM proteins and coated cultureware to meet the individual needs of your cell line. Our ECM Cell Culture Optimization Array can help you determine the best coating and concentration for your particular cell type.
» More…
Description:
Human Plasma Fibronectin Purified Protein
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 mg
Product Overview:
Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.
Key Applications:
Cell Culture
Application Notes:
Immunochemical standard
Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts
Suggested Procedure for Coating Cell Cultureware
1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.
2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.
3.Introduce the proper CO2 atmosphere, if required.
4.Add the calculated amount of HFN to each culture vessel.
5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.
6.Remove residual balanced salt solution before proceeding with standard cell culture procedures
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
View All »
UniProt Summary:
FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
SIZE: 2386 amino acids; 262607 Da
SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Inter |