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Human Amyloid β40 and Amyloid β42 Brain ELISA
Description:
Human Amyloid β40 and Amyloid β42 Brain ELISA
Qty/Pk:
96-well plate
Background Information:
Amyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s Human Amyloid β40 and Amyloid β42 Brain ELISA kits are used for the measurement of Amyloid beta 40 and Amyloid beta 42 in brain samples and for other tissue samples in a 96-well format. These assays are for research use only and appropriate for the in vitro detection of human Amyloid β40 and Amyloid β42 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts
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Applications:
This Human Amyloid β40 & Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β40 & Amyloid β42 levels in Neuroscience research.
Key Applications:
ELISA
Application Notes:
These assays require 50 µl of sample and are overnight assays.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Linearity of Dilution:
Contact technical support for information
Sensitivity:
The lowest level of Amyloid β1-40 standard used in this assay is 6.0 pg/mL (50 μL sample size).
The lowest level of Amyloid ß1-42 standard used in this assay is 8 pg/mL (50 μL sample size).
Storage Conditions:
Components in the kit can be stored up to 2 weeks at 2-8°C
Gene Symbol:
APP
Standard Curve Range:
16 pg/mL – 500 pg/mL
Product Name:
Human Amyloid β40 and Amyloid β42 Brain ELISA
Specificity:
The Amyloid β40 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-40, which causes a high selectivity for Aβ 40. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.
The Amyloid β42 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.
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Analytes Available:
Amyloid β40, Amyloid β42
Precision, %:
Intra-assay <10
Inter-assay <10
Detection Methods:
Colorimetric |