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IL-6 ELISPOT Antibody Pair, Human
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PRODUCT FAMILY INFORMATION
Elispot MultiScreenHTS Filter Plates and Antibody Pairs
The ELISPOT assay makes it possible to visualize single cells that recognize a specific structure of interest, i.e., an antigen.
The ELISPOT assay makes it possible to visualize single cells that recognize a specific structure of interest, i.e., an antigen. The sensitivity is as low as 1 out of 1,000,000 immune cells. This technique is gaining acceptance for monitoring the efficacy of vaccines for cancer and infectious diseases.
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Species Reactivity Key Applications Host Format Antibody Type
H ELISA Rat Purified Monoclonal Antibody
Description:
IL-6 ELISPOT Antibody Pair, Human
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Product Overview:
Interleukin 6 (IL-6) is a multifunctional cytokine that plays an important role in regulating immune and inflammatory responses. Produced by a wide variety of cells, including T cells, monocytes, fibroblasts, endothelial cells, and keratinocytes, IL-6 works by stimulating B-cell differentiation and antibody production, as well inducing expression of hepatic acute-phase proteins, regulating bone metabolism, and promoting neuronal differentiation. In addition, IL-6 appears to interact with IL-2 in the proliferation of T-lymphocytes and with IL-3 to promote the proliferation of multipotent hematopoietic progenitor cells. The Millipore human IL-6 ELISPOT Antibody Pair provides a sensitive and highly reliable method for the detection of IL-6 secreting cells at the single cell level.
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Specificity:
Both the capture and the detection antibodies included in this kit react with human interleukin -6 (IL-6).
Molecular Weight:
~26 kDa
Species Reactivity:
Human
Presentation:
The 500 µg purified IL-6 capture antibody is buffered in a preservative-free phosphate solution, pH 7.2, and should be handled under aseptic conditions. The 100 µg purified IL-6 biotinylated detection antibody is buffered in a phosphate solution, pH 7.2, containing 0.09% sodium azide. Streptavidin alkaline phosphatase is supplied in a Tris Buffer, pH 8.0, containing 0.1 M sodium chloride, 1 mM magnesium chloride, 0.2% (w/v) BSA, 10% (w/w) glycerol, and 0.1% sodium azide.
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Storage Conditions:
Maintain refrigerated at 2-8°C for up to six months from the date of receipt.
Components:
• Human IL-6 Capture Antibody, clone MQ2-13A5. Quantity: 500 µg.
• Human IL-6 Detection Antibody, clone MQ2-39C3. Quantity: 100 µg.
• Streptavidin Alkaline Phosphatase. Quantity: 55 μL.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
ELISA
Product Name:
IL-6 ELISPOT Antibody Pair, Human
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
1 kit
Format:
Purified
Host:
Rat
Applications:
IL-6 ELISPOT Antibody Pair, Human is a highly specific rat monoclonal antibody & that targets IL-6 & has been tested in ELISA.
Packaging:
Sufficient reagents for five, 96-well plates. |