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IL-4 ELISPOT Antibody Pair, Human
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PRODUCT FAMILY INFORMATION
Elispot MultiScreenHTS Filter Plates and Antibody Pairs
The ELISPOT assay makes it possible to visualize single cells that recognize a specific structure of interest, i.e., an antigen.
The ELISPOT assay makes it possible to visualize single cells that recognize a specific structure of interest, i.e., an antigen. The sensitivity is as low as 1 out of 1,000,000 immune cells. This technique is gaining acceptance for monitoring the efficacy of vaccines for cancer and infectious diseases.
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Species Reactivity Key Applications Host Format Antibody Type
H ELISA Mouse Purified Monoclonal Antibody
Description:
IL-4 ELISPOT Antibody Pair, Human
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Product Overview:
Interleukin-4 (IL-4) is a pleiotropic lymphoid cell growth factor that is produced by activated T cells, mast cells, and basophils. This potent cytokine works by stimulating the growth and activation of certain B cells and T cells, as well as a variety of other hematopoietic cell types, and is important for the regulation and development of T helper subsets. Based upon current literature, IL-4 has also been shown to exhibit anti-tumor activity in experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses. The Millipore human IL-4 ELISPOT Antibody Pair provides a sensitive and highly reliable method for the detection of IL-4 secreting cells at the single cell level.
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Specificity:
Both the capture and the detection antibodies included in this kit react with human interleukin -4 (IL-4).
Molecular Weight:
~15 kDa
Species Reactivity:
Human
Presentation:
The 500 µg purified IL-4 capture antibody is buffered in a preservative-free phosphate solution, pH 7.2, and the 100 µg purified IL-4 biotinylated detection antibody is buffered in a phosphate solution, pH 7.2, containing 0.09% sodium azide. Streptavidin alkaline phosphatase is supplied in a Tris Buffer, pH 8.0, containing 0.1 M sodium chloride, 1 mM magnesium chloride, 0.2% (w/v) BSA, 10% (w/w) glycerol, and 0.1% sodium azide.
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Storage Conditions:
Maintain refrigerated at 2-8°C for up to six months from the date of receipt.
Components:
• Human IL-4 Capture Antibody, clone 8D4-8. Quantity: 500 µg.
• Human IL-4 Detection Antibody, clone MP4-25D2. Quantity: 100 µg.
• Streptavidin Alkaline Phosphatase. Quantity: 55 μL.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
ELISA
Product Name:
IL-4 ELISPOT Antibody Pair, Human
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
1 kit
Format:
Purified
Host:
Mouse
Applications:
IL-4 ELISPOT Antibody Pair, Human is a highly specific mouse monoclonal antibody & that targets IL-4 & has been tested in ELISA.
Packaging:
Sufficient reagents for five, 96-well plates. |