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QCM™ Leukocyte Transendothelial
Migration Assay (Colorimetric, 24 Assays)
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PRODUCT FAMILY INFORMATION
Cell Based Assays
Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell, and cell-based activity assays. Study Apoptosis, Angiogensis, Adhesion and more.
EMD Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell and cell-based activity assays that mimic native environments for direct and indirect detection of cellular response.
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Description:
QCM™ Leukocyte Transendothelial Migration Assay (Colorimetric, 24 Assays)
Trade Name:
QCM
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here)
Introduction
Millipore’s Colorimetric QCM™ Leukocyte Transendothelial Cell Migration Assay provides an efficient model to analyze the ability of leukocytes to migrate through the endothelium. The assay is designed with a 3 µm pore size cell culture insert, appropriate for most leukocyte migration. The upper side of the cell culture insert is coated with fibronectin to support the optimal attachment and growth of endothelial cells. The assay allows investigators to evaluate the effects of various factors that influence the transendothelial process.
Precoated cell culture inserts are provided in the Millipore QCM Leukocyte Transendothelial Cell Migration Assay to significantly reduce assay time. Additionally, the assay allows quantitative analysis of leukocyte migration. Following incubation of leukocytes with the endothelial cell layer, migratory cells are stained and quantified with WST-1 reagent and measured using a spectrophotometer. Absorbance correlates with cell migration.
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Background Information:
Leukocytes patrol the vascular system. They must migrate across endothelial barriers in order to recruit to sites of inflammation. This process involves a multistep cascade consisting of leukocyte rolling, adhesion, and transmigration. A quantitative assay for leukocyte transendothelial migration has been described using a modified Boyden chamber system (Roth et al. 1995, Ding et al. 2000). The Boyden chamber system is a two chamber system with a porous membrane providing an interface between these two chambers. Endothelial cells are cultured on top of the porous membrane that is coated with an extracelluar matrix (ECM) protein. Once a confluent layer of cells is established, leukocytes are then added onto the endothelial monolayer. Leukocyte migration across the endothelium is determined by measuring the number of cells that migrate between the endothelial cells, through the porous membrane, to the lower chamber.
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Applications:
This Colorimetric QCM Leukocyte Transendothelial Cell Migration Assay provides an efficient model to analyze the ability of leukocytes to migrate through the endothelium.
Application Notes:
Results of the assay may be illustrated graphically using a bar chart to display optical density (OD) values at 420 - 480 nm.
Additional migration may also be induced or inhibited in test wells through the addition of cytokines or other pharmacological agents.
Figures 2 and 3 demonstrate typical migration results. One should use the data for reference only. This data should not be used to interpret actual assay results.
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro dia |