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α/β Integrin-mediated Cell Adhesion Array Combo Kit, fluorimetric
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PRODUCT FAMILY INFORMATION
Cell Based Assays
Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell, and cell-based activity assays. Study Apoptosis, Angiogensis, Adhesion and more.
EMD Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell and cell-based activity assays that mimic native environments for direct and indirect detection of cellular response.
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Description:
α/β Integrin-mediated Cell Adhesion Array Combo Kit, fluorimetric
Trade Name:
Chemicon (Millipore)
Qty/Pk:
2 plates
Product Overview:
Introduction
Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. A family of cell surface receptors, the integrins, mediates cell attachment to proteins of the extracellular matrix. Integrins are heterodimer molecules containing an alpha and beta transmembrane glycoprotein subunit that are non-covalently bound together (1-2). Historically, antibodies have been used to determine the integrin profiles of cells by immunoprecipitation, immunofluorescence, immunoblotting, and flow cytometry. These methods are time-consuming, laborious or require sophisticated equipment.
The CHEMICON® Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit is designed as a cost effective, efficient method for identification of cell surface integrins. The kit format uses 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The Alpha/Beta Integrin-Mediated Cell Adhesion Arrays can be completed within 1-2 hours, providing a rapid, sensitive, and quantitative assay for the characterization of alpha and beta integrin surface expression.
In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
Alpha Integrin-Mediated Cell Adhesion Array Kit (Fluorimetric) (ECM533)
Beta Integrin-Mediated Cell Adhesion Array Kit (Fluorimetric) (ECM534)
Alpha Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM530)
Beta Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM531)
Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Colorimetric) (ECM532)
QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)
QCM™ 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)
QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)
QCM™ 96-well Cell Invasion Assay (ECM555)
QCM™ 96-well Collagen-Based Cell Invasion Assay (ECM 556)
24-well Insert Cell Migration and Invasion assay systems
CytoMatrix™ Cell Adhesion strips (ECM protein coated)
QuantiMatrix™ ECM protein ELISA Kits
Test Principle
The CHEMICON® Alpha/Beta Integrin-Mediated Cell Adhesion Array Kit uses mouse monoclonal antibodies generated against human alpha (alpha1, alpha2, alpha3, alpha4, alpha5, alphaV, and alphavbeta3), and beta (beta1, beta2, beta3, beta4, beta6, alphaVbeta5, and alpha5beta1) integrins/subunits, that are immobilized onto a goat anti-mouse antibody coated microtiter plate. The plate is then used to capture cells expressing these integrins on their cell surface; goat anti-mouse antibody coated wells are provided as a negative assay control. Each strip (8 wells total) consists of 7 integrin antibody coated wells and one negative control well (see plate layout on page 4). Experimental cells are seeded onto the coated surface and incubated. Subsequently, unbound cells are washed away, and the adherent cells are lysed and detected by the patented CyQuant GR dye (Molecular Probes) (3-4). Th |