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QCM™ Endothelial Cell Invasion Assay (24 well, fluorometric)
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PRODUCT FAMILY INFORMATION
Cell Based Assays
Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell, and cell-based activity assays. Study Apoptosis, Angiogensis, Adhesion and more.
EMD Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell and cell-based activity assays that mimic native environments for direct and indirect detection of cellular response.
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Description:
QCM™ Endothelial Cell Invasion Assay (24 well, fluorometric)
Trade Name:
QCM
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here)
Introduction
Endothelial cells (EC) invade through the basement membrane (BM) to form sprouting vessels. The invasion process consists of the secretion of matrix metalloproteases (MMP) to degrade basement membrane, the activation of endothelial cells, and the migration of EC across the basement membrane. The understanding of EC invasion is important for studying the mechanism of angiogenesis in injured tissue as well as in disease such as cancer.
Cell migration may be evaluated through several different methods, the most widely accepted of which is the Boyden Chamber assay. The Boyden Chamber system uses two-chamber system which a porous membrane provides an interface between two chambers. Cells are seeded in the upper chamber and chemoattractants placed in the lower chamber. Cells in the upper chamber migrate toward the chemoattractants by passing through the porous membrane to the lower chamber. Migratory cells are then stained and quantified.
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Applications:
This QCM Endothelial Cell Invasion Assay provides an in vitro model to quickly screen factors that can regulate endothelial invasion.
Application Notes:
Millipore’s QCM Endothelial Cell Invasion Assay provides an in vitro model to quickly screen factors that can regulate endothelial invasion. The assay is performed in an invasion chamber using a basement membrane protein coated on the porous insert. The level of coating and the pore size is optimized for endothelial cells so the researcher may utilize this kit to mimic physiological condition. After cell invasion has occurred, the researcher can select a staining method to quantify the number of cells that have invaded through the chamber. Millipore offers a colorimetric staining kit (ECM210) and a fluorometric kit staining reagent (ECM211) for both convenience and efficiency.
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Components:
ECM211-1
1. 24-well Cell Invasion Plate Assembly: (Part No. CS203020) Two 24-well plates each containing 12 ECMatrix™-coated 3 µm inserts per plate.
2. 24-well Stain Extraction Plate: (Part No. 2005871) One each.
3. 96-well Quantitation Plate: (Part No. CR201763) One each.
4. Forceps: (Part No. 10203) One each.
ECM211-2
1. Cell Detachment Solution: Accutase™ (Cat. No. SCR005) One 100 mL bottle.
2. Calcein-AM: (Part No. CS202541) One 50 µg vial.
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Storage Conditions:
ECM211-1 kit components should be stored at 2° to 8°C up to the expiration date provided on the kit. DO NO |