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Millicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105
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PRODUCT FAMILY INFORMATION
Fibronectin Antibodies, Proteins & Assays
Millipore’s Fibronectin Antibodies, proteins, and assays are based on the expertise of Upstate & Chemicon. See below products related to Fibronectin research, including Migration assays & ELISA kit.
EMD Millipore’s Fibronectin related Antibodies, proteins and assays are based on the expertise of Upstate & Chemicon. All have been well validated and published. See below for data, references and related products for Fibronectin.
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Cell Based Assays
Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell, and cell-based activity assays. Study Apoptosis, Angiogensis, Adhesion and more.
EMD Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell and cell-based activity assays that mimic native environments for direct and indirect detection of cellular response.
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ECM Proteins and Coated Plates
Meet cell type-specific needs for growth and adhesion
Millipore offers a wide variety of ECM proteins and coated cultureware to meet the individual needs of your cell line. Our ECM Cell Culture Optimization Array can help you determine the best coating and concentration for your particular cell type.
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Description:
Millicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 set
Product Overview:
Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion and migration of many diverse cell types on various extracellular matrix (ECM) component proteins. Millicoat™ Cell Adhesion Strips are provided as 12 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The wells in rows A - G have been coated with a human ECM protein. Row H of each strip is coated with BSA which serves as a negative assay control. The MillicoatTM screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I and collagen IV. Cells are seeded onto the coated substrate. Subsequently, adherent cells are fixed and stained. Relative attachment is determined using absorbance readings.
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Applications:
The MillicoatTM screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I & collagen IV.
Key Applications:
Activity Assay
Application Notes:
PROCEDURE:
NOTE: Optimal assay performance is obtained using subconfluent cell cultures. This can be achieved by splitting the cells 1 to 2 days prior to performing the assay.
1. Rehydrate the strips with 200 mL of PBS per well for at least 15 minutes at room temperature. Remove the PBS from the rehydated strips.
2. Prepare a single cell suspension, preferably using a non-enzymatic dissociation buffer. Optimum cell density may be determined by titration of the cells. A common starting range is between 1x10E05 to 1x10E07 cells/mL.
3. Add 100 mL of the diluted cell suspension to each well. Incubate the plate at 37°C for 1 hour in a CO2 incubator. Gently wash the plate 3 times with PBS containing Ca2+/Mg2+ (200 mL/well).
4. Add 100 mL/well of 0.2% crystal violet in 10% ethanol to each well. Incubate for 5 minutes at room temperature. Remove the stain from the wells. Gently wash the plate 3 times with PBS (300 mL/well).
5. Add 100 mL of Solubilization Buffer (A 50/50 mixture of 0.1M NaH2PO, pH 4.5 and 50% ethanol) to each well. Allow strips to incubate and gentl |