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JNK 1/2 STAR ELISA Kit
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PRODUCT FAMILY INFORMATION
STAR ELISA Kits
Upstate STAR (Signal Transduction Assay Reaction) ELISA kits are a fast, sensitive method to detect the relative amounts of total and activated targets through the use of phospho-specific antibodies.
Upstate STAR (Signal Transduction Assay Reaction) ELISA kits are a fast, sensitive method to detect the relative amounts of total and activated targets through the use of phospho-specific antibodies.
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Description:
JNK 1/2 STAR ELISA Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
96 assays
Background Information:
test Principle
The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The JNK 1/2 plate is coated with a specific mouse monoclonal JNK 1/2 capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing JNK 1/2 antigen to be captured in the plate wells. The plate is then washed to remove any non-bound unspecific material. The wells are then incubated with a specific rabbit anti- JNK 1/2 antibody to detect the captured JNK on the plate well. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard line of detection.
JNK Background
JNK (c-Jun N-terminal Kinase, MAPK8) is a member of the MAP kinase family. JNK was originally found to be stress activated, and as such are also referred to as the stress-related MAPK. Four alternatively spliced transcript variants encoding distinct isoforms of JNK have been reported. JNK is regulated by stress inducing signals such as osmotic stress and UV radiation as well as proinflammatory cytokines such as IL-1 and TNFα. Just as Erk (p44/p42, MAPK) is activated by its upstream kinase MEK, JNK is phosphorylated and activated by MKK4 and MKK7 on the quintessential MAPK activation sequence of Thr-X-Tyr, where X is a Pro for the JNK kinases on Thr183/Tyr185 for JNK1 and Thr221/Tyr223 for JNK2. Once phosphorylated and activated, JNK goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. JNK serves as a transcriptional regulator and is known to phosphorylate a number of downstream targets, including the transcription factors c-Jun, ATF-2, and ELK-1. C-Jun, a member of the AP-1 family of transcription factors, regulates cytokine gene expression. Along these lines, JNK inhibitors may be effective in the control of rheumatoid arthritis (RH), an autoimmune disease involving increased production of inflammatory cytokines. Inhibition of JNK signaling also enhances chemotherapy-induced inhibition of tumor cell growth, suggesting the JNKs may provide a molecular target for the treatment of cancer. Both JNK and p38 MAPK are often simultaneously activated in response to environmental changes. These two stress kinase signaling pathways have evolved to relay increasingly complex ranges of environmental stimuli. The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. The cell-permeable JNK inhibitory peptide may have promise as a therapeutic agent for diabetes.
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Applications:
The JNK 1/2 colorimetric STAR ELISA kit is a solid phase sa |