|
phospho-Akt (Thr308) STAR ELISA Kit
Close
PRODUCT FAMILY INFORMATION
Phospho-Akt Antibodies, Kits & Assays
Millipore’s phosphoAkt antibodies have been well validated and published. See below or a comprehensive list of our phosphoAkt products, based on the expertise of Upstate & Chemicon.
EMD Millipore’s phosphoAkt antibodies are based on the expertise of Upstate & Chemicon. Our phosphoAkt antibodies have been well validated and published. See the ordering tab for a comprehensive list of our phosphoAkt products and direct links to their technical pages for data, references and related products.
» More…
STAR ELISA Kits
Upstate STAR (Signal Transduction Assay Reaction) ELISA kits are a fast, sensitive method to detect the relative amounts of total and activated targets through the use of phospho-specific antibodies.
Upstate STAR (Signal Transduction Assay Reaction) ELISA kits are a fast, sensitive method to detect the relative amounts of total and activated targets through the use of phospho-specific antibodies.
» More…
Description:
phospho-Akt (Thr308) STAR ELISA Kit
Trade Name:
STAR ELISA
Upstate (Millipore)
Qty/Pk:
96 assays
Background Information:
The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The AKT plate is coated with a specific mouse monoclonal anti-AKT capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing AKT antigen to be captured in the plate wells. The plate is then washed to remove any non-bound unspecific material. The wells are then incubated with a specific rabbit anti-AKT antibody to detect the captured phospho-AKT (Thr308) on the plate well. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard curve.
II. Akt BACKGROUND
Akt (Protein Kinase B), a Ser/Thr kinase, is a major known effecter of the PI3 Kinase pathway and is involved in multiple signaling pathways that relate to many biological processes including glucose metabolism, cell survival/apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. Akt is activated by ligand-stimulated growth factor receptor signaling that activates the Phosphatidylinositol 3-kinase (PI3 Kinase, PI3K) dependent manner. PKB is one of the most frequently hyperactivated protein kinases in human cancers. In mammals three isoforms of Akt (Akt1/PKBα, Akt2/PKBβ, and Akt3/PKBγ) exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and post-natal survival. Additionally, Akt1/PKBα is required for adipocyte differentiation, where as Akt2/PKBβ and Akt3/PKBγ are not. Akt2 is strongly correlated with the regulation of glucose homeostasis and is the predominant PKB isoform expressed in insulin-responsive tissues where defective Akt2 results in impaired insulin-stimulated glucose uptake in muscle and adipocytes. Akt3 is abundant in br |